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Neuroscience

When you are studying central neurotransmission, the effects of drugs and disease, you need precise, reproducible results, and you need them fast.The continuing need for greater temporal and spatial resolution—often with lower-volume microdialysis perfusate samples—requires the use of extremely sensitive analytical instrumentation. Thermo Scientific Dionex LC systems for neuroscience offer distinct benefits to help you get the utmost information from your precious samples.

Using these LC systems and our highly sensitivity and selective detectors, along with proven analytical methods, will help you to:

  • Measure femtogram levels of analytes
  • Analyze multiple neurotransmitters simultaneously
  • Conserve precious samples and reagents using UHPLC
  • Increase sample throughput with reliable, maintenance-free sensors

Monoamines and Metabolites

Whether you are investigating levels of biogenic amines in tissue samples, from cell cultures or microdialysis perfusates, the Thermo Scientific Dionex UltiMate 3000 Electrochemical detector is optimized for routine, high-sensitivity analysis of monoamines and their metabolites. With our high sensitivity electrochemical detection technology, dopamine (DA) and serotonin (5HT) levels below 50 fg can be achieved with analysis time less than two minutes – the ideal solution to markedly improve microdialysis temporal resolution.

Acetylcholine

The study of cholinergic neurotransmission in vivo requires a stable, easy-to-use approach.  With the addition of an in-line, solid-phase reactor, the UltiMate 3000 Electrochemical detector achieves sensitivity levels as low as 5 pg for acetylcholine (ACh) in a microdialysis sample.

Neuroactive Amino Acids

Neuroactive amino acids act as excitatory and inhibitory molecules in the CNS. Measurement of low levels of these amino acids from basal striatal microdialysis perfusates can be accomplished in under five minutes. Achieving this fast turnaround time is simplified using our fully automated UltiMate UHPLC system with the UltiMate 3000 electrochemical detector and the 6011RS ultra analytical cell with unique dual coulometric sensors and solid-state reference electrodes.

Free D-Serine and D-Aspartic Acid in Tissue

Once thought only to be common in lower organisms, D amino acid enantiomers can now easily be quantitated from mammalian tissue homogenates, even in the presence of large amounts of their corresponding L-enantiomers. Our fast UHPLC method involves simple sample extraction, followed by automated precolumn derivatization for femtogram level detection with the Thermo™ Scientific™ Dionex™ UltiMate 3000 Fluorescence detector.  This approach provides enhanced resolution beyond other HPLC methods.

Multiple Neurochemical Profiling

To fully understand the functioning of a particular neurotransmitter pathway it is important to not only be able to measure the neurotransmitter itself, but also its precursors and catabolites.  Target specific neurotransmitters and metabolites or perform metabolomic studies using the unique Thermo™ Scientific™ Dionex™ CoulArray™ Multi-electrode array electrochemical detector.