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Protein Analysis

Newly discovered and purified proteins typically undergo rigorous structural and functional characterization. Structural characterization may include peptide mapping, amino acid sequence determination through de novo sequencing, or identification using a mass spectrometer. The three-dimensional structures of proteins are typically determined by X-ray crystallography and NMR spectroscopy.

Many proteins also undergo posttranslational modifications (PTMs) after their synthesis. PTMs extends the functionality of the protein by adding functional groups such as acetate, phosphate, various lipids, and carbohydrates, thereby changing the chemical nature of an amino acid or by making structural changes, like the formation of disulfide bridges. Proteins are also modified through degradation processes like deamidation or through human engineering. The detection, characterization, and quantification of PTMs and other modifications are mandated by FDA and other regulatory agencies where protein products are used as therapeutics.

Dionex offers next generation analytical HPLC systems, combined with an outstanding array of column innovations that addresses every facet of protein analysis and characterization. High-resolution HPLC systems required in nano scale separation for peptide mapping and LC/MS, to the research scientists that demand highest application flexibility to highly specialized and regulated analytical methods employed in the QA/QC of recombinant protein and monoclonal antibody therapeutics where robustness, reliability and throughput is considered absolutely necessary.