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RSLCnano Autosampler

The WPS-3000 Well Plate Autosampler has been specifically designed to provide unattended sample throughput, combined with highly reproducible injections of even the smallest samples volumes. The RLSCnano Well Plate Sampler extends these capabilities to pressures up to 1000 bar. It is the ideal solution for chromatography users looking to work with the highest injection precision at nano, capillary, and micro flow rates in UHPLC applications.

The sample rack handles up to three well plates (96 or 384 wells—sealed or unsealed) or sample vial trays, or combinations thereof. Sample plates can be exchanged flawlessly during a run. The cooling option cools the samples down to 4 °C, preserving thermolabile samples.

  • Three well plate capacity (up to 1152 samples)
  • Injection valve pressure stability up to 1000 bar
  • Patented sample thermostatting, 4–45 °C, 22 °C below ambient
  • Sealed sample compartment for outstanding temperature stability
  • Allows injections of thermolabile proteins
  • Dual-needle injection principle
  • Large injection range: 20 nL to 125 µL
  • Unmatched injection precision
  • Lowest carryover: typically <0.01%

Injection Mechanism

 

 

Using the dual-needle principle, the vial septum of the well seal is pierced by the stainless steel outer needle. The sampler guides the sampling needle, made of a fused silica capillary, through the piercing needle down to the bottom of the sample carrier. Sample needle height is easily programmed to match sample vial or well depth. Injections of minute sample volumes, with virtually zero sample loss—1 µL injection per 1 µL sample—are performed using the µL-pickup routine. This routine is of particular importance when injecting protein samples that cannot be diluted without the risk of protein loss or alteration.

In order to guarantee the best chromatographic performance when injecting volumes in the ranges of 20 nL–250 µL, the WPS‑3000 works with a pulled loop injection mechanism. This dramatically reduces delay times in the system and provides more accurate and precise gradient profiles, reduces peak broadening, and allows for better peak resolution.

Order Information

Call 1-800-DIONEX-0 (zero) for order information.