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LC Front-End for MS and MS/MS

The cost of mass spectrometers sets high expectations for LC productivity and sample throughput. As a front-end, the UltiMate® 3000 delivers high productivity and performance across the full range of sample sizes, column dimensions, and flow rates to meet the growing diversity of MS applications. The UltiMate 3000 optimizes separations for all your LC/MS applications, providing a new level of productivity and versatility.

Work With Advanced UltiMate 3000 Technology

The UltiMate 3000 series is a versatile front-end to mass spectrometers. From classical binary to quaternary and dual-gradient micro systems, all the benefits provided by this technology are immediately available to users. This modular flexibility significantly improves performance and productivity.

  • RSLCnano x2 dual system
  • Splitless flow generation from 20 nl/min to 50 µl/min at column pressures up to 800 bars
  • Continuous, interruption free flow delivery
  • Can be converted easily from nano to capillary to micro applications

Binary Rapid Separation System

  • Best flow rate and gradient precision even at very low HPLC flow rates
  • Variable pump mixing volume for shortest gradient delay volume or improved eluent mixing
  • Support of small particle column packings with system pressures up to 800 bar (11,600 psi)
  • Very fast cycle times; gradient delay volumes <100 µL and injection cycle times <15 s
  • Accurate, precise flow and gradient delivery through patented SmartFlow technology

Quaternary and x2 Dual Micro Flow Systems

  • Four eluents for maximum flexibility and fast method development
  • Reduced gradient delay volumes of <350 µL, allows quaternary pumps to be used as MS front-ends
  • Superior eluent mixing (compared to binary systems) provides better peak shapes and higher resolution

Solutions for Your LC-MS Applications:

  • Use LC separations to overcome suppression effects or isomeric interferences
  • Protein purification, characterization, and identification.
  • Increased sample throughput for greater return on your MS detector investment
  • 2-Dimensional liquid chromatography for higher selectivity and resolution

Optimizing MS Applications

UltiMate 3000 RSLC systems are ideal for microbore applications that dominate LC-MS applications. Delay volume has been reduced to an absolute minimum—less than 100 µL. Run fast, precise gradient separations with the shortest possible cycle times in single-pump or dual-pump configurations. By utilizing either a precolumn or postcolumn active splitter, the UltiMate 3000 can deliver the ideal flow rate to your MS independent of the flow range (nano through to semipreparative).

Maximum Usability and Flexibility

All UltiMate 3000 systems are controlled directly from Xcalibur (Thermo Fisher Scientific), Analyst® (ABI/Sciex), and HyStar (Bruker Daltonics). The MS workstation sets up the sample sequences, processes the mass spectrometry data, and controls all LC modules. Realize the full benefit of the UltiMate 3000 single- and dual-pump systems without having to learn (or purchase) a new software control application.