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UltiMate 3000 Rapid Separation LC Systems

The Thermo Scientific Dionex UltiMate 3000 Rapid Separation LC (RSLC) systems integrate unique hardware features for ultrafast separations and excellent resolution on columns with small particles. In addition, they are characterized by a maximum level of reliability and flexibility. For more product information please visit the UltiMate™ 3000 UHPLC systems and solutions webpage.

  • Thermo Scientific Dionex SmartFlow pulsation-free flows at flow rates up to 8 mL/min at pressures up to 15,000 psi (103.4 MPa).
  • High-pressure or low-pressure gradient proportioning
  • Easily adjustable system gradient delay volume supporting a wide range of LC applications
  • In-line split-loop injections for 15 second, no-sample-loss injections
  • Column compartment temperatures up to 110 °C for reduced system backpressure
  • Data collection rates up to 200 Hz with a wide range of optical detectors including three UV variants and fluorescence
  • Full UV spectra data even at 200 Hz (diode array detection)
  • Run both ultrafast separations and conventional LC methods

The UltiMate 3000 RSLC systems allow you to work over an extensive flow-pressure footprint from 100 µL/min to 8 mL/min (RSLC) or 20 nL/min to 2.5 mL/min (RSLCnano) and 800 bar. This covers the full range of HPLC or nano HPLC applications, including conventional and ultrafast LC, on one system platform.

The Thermo Scientific Dionex Rapid Separation Autosampler allows robust 500 μL injections over the full backpressure range. In addition, it handles a multitude of different sample formats and supports a maximum of 1167 samples. The Thermo Scientific Dionex RLSCnano Wellplate Sampler extends these capabilities to pressures up to 1000 bar over a wide injection range, from 20 nL to 125 µL.

The RSLC system column compartment can be upgraded easily and converted with different column switching valves. The compartment handles up to 12 columns (depending on column dimensions) up to 30 cm length. UV detectors come with a wide variety of different flow cells. The NCS-3500RS module for the Thermo Scientific Dionex RSLCnano system provides a dual-gradient pump and column compartment in one housing. This unique combination allows you to tune your separation for the highest resolution or the fastest analysis time, all within one module.

Separation of 10 compounds in 10 seconds, with a full 100 Hz DAD spectral scan. Flow rate: 3.7 mL/min, backpressure: 730 bar, oven temperature: 100 °C.

Ultimate Speed and Performance

The Ultimate 3000 RSLC systems in combination with the Thermo Scientific Dionex UltiMate 3000 Rapid Separation Diode Array Detector support peak characterization of the sharpest peaks of ultrafast separations, for reliable sample data. No matter if you run RSLC or conventional LC methods, the UltiMate 3000 RSLC systems will convince you with their ultimate performance. Use the method transfer calculator for quick and easy conversion of conventional LC to RSLC methods.

Do you need shorter run times for high sample throughput or instant results in process analyses? Does your current LC method lack resolution, and do you want to generate higher peak capacity? Then learn more about the benefits which are provided by the RSLC system in combination with Thermo Scientific Acclaim RSLC columns.

The RSLC system provides excellent retention time precision for both conventional LC (A) and UHPLC (B) separations (gradient application).

UltiMate 3000 Binary Rapid Separation LC System

The UltiMate® 3000 Binary Rapid Separation LC (RSLC) system is the perfect choice for developing the fastest UHPLC separations possible.

UltiMate 3000 Quaternary Rapid Separation LC System

The UltiMate® 3000 Quaternary Rapid Separation LC (RSLC) system is the ideal combination of quaternary eluent proportioning and RSLC technology.

UltiMate 3000 x2 Dual Rapid Separation LC System

The UltiMate® 3000 x2 Dual Rapid Separation LC (RSLC) system combines the ultrahigh speed and resolution of RSLC with x2 dual technology. Make use of advanced chromatographic techniques, even at ultrahigh speed.

Introducing the Dionex UltiMate 3000 BioRS from Thermo Scientific

Developed for reliability and robustness under harsh solvent conditions, the UltiMate™ 3000 BioRS system brings highly flexible UHPLC separation capabilities to a wide range of biopharmaceutical research and QA/QC analyses.

UltiMate 3000 RSLCnano System

The UltiMate 3000 RSLCnano system combines the separation power of RSLC and the sensitivity of nano LC. The ideal solution for high-resolution separation in LC-MS proteomics.